Species:canine

Tissue:leg

Cell type:cell line

Morphology:

Growth properties:Adherent

Complete medium:PETCC medium-B

Culture environment:37 ℃，5% CO₂，95% air

Subculturing renewal:every other day

Subculturing ratio:standard trypsinization followed by 1/4 dilution

Use restriction:only for scientific research，not for use in human body

Tumorigenic:

Receiving suggestions:After you sign for the cell, wipe the surface of the T25 flask with 75% alcohol, but do not open the cap of the culture flask, and observe the cell state under the microscope. whereupon, it is recommended that you place the flask flat in the incubator for 2 to 4 hours to balance and rewarm, conduct cell passage when the cell density is greater than or equal to 80% (note: do not cultivate with high density or high density for a long time, which may lead to enhanced cell adhesion); For the first subculturing, it is recommended you conduct standard trypsinization followed by 1:2 dilution, with retention of part of the original medium helping the cells to adapt and transition.

Cryopreservation:PETCC cryopreservation medium-B

Storage conditions:Liquid nitrogen

Provider: